LncRNA MBNL1-AS1 represses cell proliferation and enhances cell apoptosis via targeting miR-135a-5p/PHLPP2/FOXO1 axis in bladder cancer.

摘要: LncRNAs have been shown to play essential roles in bladder cancer (BC) progress. Our microarrays of clinical samples firstly screened that lncRNA muscleblind-like 1 antisense RNA (MBNL1-AS1) was poorly expressed BC tissues. However, its biological function remains not well understood. Here we examined the correlations with MBNL1-AS1 patients. Then, 5673 and T24 cell lines were employed investigate role proliferation apoptosis cells vitro vivo. Furthermore, miR-135a-5p (miR-135a)/PHLPP2/FOXO1 axis focused explore regulatory mechanism BC. The results showed significantly downregulated tumor tissues, associated progression. In vitro, knockdown increased number viable bromodeoxyuridine-positive cells, accelerated cycle, dysregulated proliferative regulators (Ki67, p21, p27, Cyclin D1) cells. apoptotic cleavages caspase-3/9 reduced MBNL1-AS1-silenced Overexpression had opposite effects on apoptosis. Moreover miR-135a demonstrated interact MBNL1-AS1, inhibiting reversed shMBNL1-AS1 downstream effectors (PHLPP2 FOXO1) positively regulated by but negatively miR-135a. Similar also observed xenograft tumors. conclusion, this study suggests acts as a suppressor targeting miR-135a/PHLPP2/FOXO1 axis, providing novel insight for diagnosis treatment.