Dual initiation sites of protein synthesis on foot-and-mouth disease virus RNA are selected following internal entry and scanning of ribosomes in vivo.

摘要: The initiation of protein synthesis on foot-and-mouth disease virus RNA occurs at two sites separated by 84 nucleotides. Immediately upstream from the first these is internal ribosome entry site (IRES), which directs translation this to be cap-independent. utilization has been examined using artificial fusion genes in vivo under a variety conditions. Additional in-frame AUG codons have introduced between authentic start determine mechanism ribosomes recognize second site. results indicate that following 5' side codon, many fail initiate position and scan along In presence or absence IRES both are efficiently used but slightly biased towards IRES. Furthermore, IRES, initiates independently activity cap-binding complex.