[18] l-kynurenine transaminase from Hansenula schneggii

摘要: Publisher Summary This chapter provides an overview of L-kynurenine transaminase from Hansenula schneggii. L - Kynurenine α ketoglutarate aminotransferase catalyzes the transfer -amino group to α-ketoglutarate yield L-glutamate and o-aminobenzoylpyruvic acid, which is immediately converted intramolecularly dehydrated cyclized form, kynurenic acid. The describes assay method, purification procedures, general properties L-kynurenine-α-ketoglutarate yeast, method routinely employed based on spectrophotometric determination acid formed. L-Kynurenine consumption determined by measuring rate decrease in absorbance at 370 nm because deamination L-kynurenine. procedure consists several steps comprising preparation crude extract, polyethyleneimine treatment, DEAE-cellulose column chromatography, BioGel P-300 chromatography. Approximately 930-fold achieved with overall 40%.