Experimental transmission of bovine leukosis virus by simulated rectal palpation.

摘要: Eight six-month-old Holstein male calves were experimentally inoculated by rectal palpation with whole blood from a donor seropositive to bovine leukosis virus. The inoculation consisted of the deposition 2 ml on disposable obstetrical sleeve followed 30 second simulate process pregnancy detection or artificial insemination. This procedure was repeated at weekly intervals for three consecutive weeks. All eight developed antibodies virus within five weeks after initial palpation. presence demonstrated in peripheral leucocytes all nine These results indicated that routine may be an effective mode spread susceptible cattle.