Detecting parasitised eggs with polymerase chain reaction and DNA sequence of Trichogramma australicum Girault (Hymenoptera: Trichogrammatidae)
作者： W Amornsak , G Gordh , G Graham
DOI: 10.1111/J.1440-6055.1998.TB01567.X
关键词:
摘要: The nuclear internal transcribed spacer 2 (ITS2) regions of the egg parasitoid Trichogramma australicum Girault, cotton bollworm Helicoverpa armigera, (Hubner) and punctigera (Wallengren) were amplified by polymerase chain reaction (PCR) to yield 408, 588 644 b.p. fragments, respectively. DNA sequence information was used design unique primers for specific amplification T. from individual parasitised eggs. australicum-specific PCR products first visible 12h after eggs parasitised. This assay can be monitor wasp populations under field conditions.
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