Upregulated microRNAs in membranous glomerulonephropathy are associated with significant downregulation of IL6 and MYC mRNAs.

摘要: Membranous glomerulonephropathy (MGN) is a glomerulopathy characterized by subepithelial deposits of immune complexes on the extracapillary side glomerular basement membrane. Insertion C5b-9 (complement membrane-attack complex) into membrane leads to functional impairment capillary wall. Knowledge molecular pathogenesis MGN actually scanty. MicroRNA (miRNA) profiling in and unaffected tissues was performed TaqMan Low-Density Arrays. Expression miRNAs miRNA targets evaluated Real-Time polymerase chain reaction (PCR). In vitro transient silencing achieved through transfection with inhibitors. Ten (let-7a-5p, let-7b-5p, let-7c-5p, let-7d-5p, miR-107, miR-129-3p, miR-423-5p, miR-516-3p, miR-532-3p, miR-1275) were differentially expressed (DE) biopsies compared controls. Interleukin 6 (IL6) MYC messenger RNAs (mRNAs; DE miRNAs) significantly downregulated from patients, upregulated A498 cells following let-7a-5p or let-7c-5p silencing. Gene ontology analysis showed that regulate pathways associated pathogenesis, including cell cycle, proliferation, apoptosis. A significant correlation between mRNAs clinical parameters (i.e., antiphospholipid antibodies, serum creatinine, estimated filtration, proteinuria, cholesterol) has been detected. Based our data, we propose their downstream network may be involved could considered as potential diagnostic biomarkers MGN.