Key regulatory oxysterols in liver: analysis as Δ4-3-ketone derivatives by HPLC and response to physiological perturbations

摘要: A number of oxysterols have been implicated in metabolic regulation. Key among these are (24S),25-epoxycholesterol and (24S)-hydroxycholesterol, high affinity ligands for the nuclear transcription factor liver X receptor alpha; 27-hydroxycholesterol, a bile acid synthetic intermediate; 25-hydroxycholesterol, which has used to study regulation lipid metabolism by sterol regulatory element-binding protein family factors. Investigation physiological importance compounds vivo hampered lack analytical methods reproducibly accurately determine their concentrations tissues. This article describes method designed quantitatively amounts important side-chain derivatization Delta4-3-ketones followed performance liquid chromatography. The was validated with known standards then rodent under various conditions. All four were present picogram per milligram range distinct subcellular distributions responses perturbations vivo.