Amperometric Quantification of NADH Based on Graphene/Methylene Blue Nanocomposite Thin Films on Au(111)

摘要: Graphene (Gr)/methylene blue (MB) composites were successfully prepared and amperometric detection of nicotinamide adenine dinucleotide (NADH) was studied on the Gr/MB composite thin films modified Au(111) electrode. Gr sheets fabricated by using two-step electrochemical approaches: intercalation sodium dodecyl sulfate (SDS) into graphite working electrode electrooxidation exfoliation SDS-intercalated reduction to obtain Gr/SDS suspension. In order prepare structure, first SDS removed from suspension then MB added dispersion ultrasonicated, vacuum-filtered through a membrane, re-dispersed water. nanocomposite supported substrates drop-casting this dispersion. Characterization carried out scanning electron microscopy (SEM), dispersive spectroscopy (EDS), UV–Visible absorption spectroscopy, Fourier transform infrared (FTIR), cyclic voltammetry techniques. Gr-MB/Au(111) electrode, having an excellent electrocatalytic activity, used for determination NADH. The oxidation current density linearly proportional NADH concentration in range between 1.0 264 μM. sensitivity sensor calculated as 0.316 µA µM−1 cm−2 with limit 0.3 µM at signal-to-noise ratio 3.0. POLYM. COMPOS., 2016. © 2016 Society Plastics Engineers