Overexpression in Escherichia coli of 12 Vitamin B12 Biosynthetic Enzymes

作者: C.A. Roessner , J.B. Spencer , S. Ozaki , C.H. Min , B.P. Atshaves

DOI: 10.1006/PREP.1995.1019

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摘要: The first 12 enzymes involved in the biosynthesis of vitamin B12 from its five-carbon precursor, aminolevulinic acid, have been overexpressed recombinant strains Escherichia coli. activity each enzyme has demonstrated by hydrogenobyrinic acid acid.

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