Behaviors of Leucine and Isoleucine in Ion Mobility‐Quadrupole Time of Flight Mass Spectrometry
作者： Su Guo , Fang Zhang , Haoyang Wang , Manyu Zhang , Zhixu Zhang
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摘要: In this study, ion mobility separation coupled with tandem mass spectrometry (IM-tandem MS) was utilized to investigate the ionization behaviors of two amino acids including leucine and isoleucine. Under electrospray (ESI) mode, protonation sites in each molecular sturcture caused forms protomer. One arose from being protonated (amino-protomer) other carboxyl (carboxyl-protomer). two-dimensional (drift time, m/z) spectrum, protomers had same mass, but distinguishable drift times fragmentation patterns. For characterization purpose, theoretical collision cross section (CCS) values were calculated proven be consistent experimental. Moreover, quantified relationship between their evaluated. It showed that abundance carboxyl-protomer proportional concentration acid, whereas amino-protomer did not have trend. atmospheric pressure chemical (APCI) only observed. addition, cluster ions observed under ESI absent completely. The results demonstrate mode impacts heavily on isoleucine form therir also relationship.
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