Utility of Helicobacter spp. associated GFD markers for detecting avian fecal pollution in natural waters of two continents.
作者： W. Ahmed , V.J. Harwood , K. Nguyen , S. Young , K. Hamilton
DOI: 10.1016/J.WATRES.2015.10.050
关键词:
摘要: Avian fecal droppings may negatively impact environmental water quality due to the presence of high concentrations indicator bacteria (FIB) and zoonotic pathogens. This study was aimed at evaluating performance characteristics utility a Helicobacter spp. associated GFD marker by screening 265 wastewater samples from range avian non-avian host groups two continents (Brisbane, Australia Florida, USA). The host-prevalence -specificity this among tested Brisbane were 0.58 0.94 (maximum value 1.00). These values for Florida 0.30 (host-prevalence) 1.00 (host-specificity). markers in nucleic acid measured test concentration 10 ng laboratories using quantitative PCR (qPCR) assay. mean (5.2 × 103 gene copies) orders magnitude higher than (8.6 101 copies). evaluated testing River, freshwater creek Florida. Among 18 83% (n = 18) positive marker, ranged 6.0 101–3.2 102 copies per 100 mL water. In all, 92% 25) also with ranging 2.8 101–1.3 104 Based on results, it can be concluded that is highly specific groups, could used as reliable detect amount pollution waters.
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Valerie J. Harwood, Bruce Wiggins, Charles Hagedorn, R. D. Ellender, Jan Gooch, James Kern, Mansour Samadpour, Annie C. H. Chapman, Brian J. Robinson, Brian C. Thompson, Phenotypic library-based microbial source tracking methods: efficacy in the California collaborative study. Journal of Water and Health. ,vol. 1, pp. 153- 166 ,(2003) , 10.2166/WH.2003.0018
David M Gordon, Sonja Bauer, James R Johnson, The genetic structure of Escherichia coli populations in primary and secondary habitats. Microbiology. ,vol. 148, pp. 1513- 1522 ,(2002) , 10.1099/00221287-148-5-1513
G. H. Reischer, D. Kollanur, J. Vierheilig, C. Wehrspaun, R. L. Mach, R. Sommer, H. Stadler, A. H. Farnleitner, Hypothesis-Driven Approach for the Identification of Fecal Pollution Sources in Water Resources Environmental Science & Technology. ,vol. 45, pp. 4038- 4045 ,(2011) , 10.1021/ES103659S
W. Ahmed, J. P. S. Sidhu, S. Toze, An attempt to identify the likely sources of Escherichia coli harboring toxin genes in rainwater tanks. Environmental Science & Technology. ,vol. 46, pp. 5193- 5197 ,(2012) , 10.1021/ES300292Y
C. Andrew Carson, Jessica M. Christiansen, Helen Yampara-Iquise, Verel W. Benson, Claire Baffaut, Jerri V. Davis, Robert R. Broz, William B. Kurtz, Wendi M. Rogers, William H. Fales, Specificity of a Bacteroides thetaiotaomicron Marker for Human Feces Applied and Environmental Microbiology. ,vol. 71, pp. 4945- 4949 ,(2005) , 10.1128/AEM.71.8.4945-4949.2005
W. Ahmed, C. Wan, A. Goonetilleke, T. Gardner, Evaluating sewage-associated JCV and BKV polyomaviruses for sourcing human fecal pollution in a coastal river in Southeast Queensland, Australia. Journal of Environmental Quality. ,vol. 39, pp. 1743- 1750 ,(2010) , 10.2134/JEQ2010.0062
Beverly J. Kildare, Christian M. Leutenegger, Belinda S. McSwain, Dustin G. Bambic, Veronica B. Rajal, Stefan Wuertz, 16S rRNA-based assays for quantitative detection of universal, human-, cow-, and dog-specific fecal Bacteroidales: A Bayesian approach Water Research. ,vol. 41, pp. 3701- 3715 ,(2007) , 10.1016/J.WATRES.2007.06.037
Orin C. Shanks, Catherine A. Kelty, Shawn Archibeque, Michael Jenkins, Ryan J. Newton, Sandra L. McLellan, Susan M. Huse, Mitchell L. Sogin, Community structures of fecal bacteria in cattle from different animal feeding operations. Applied and Environmental Microbiology. ,vol. 77, pp. 2992- 3001 ,(2011) , 10.1128/AEM.02988-10
Johan Åström, Thomas J. R. Pettersson, Georg H. Reischer, Tommy Norberg, Malte Hermansson, Incorporating expert judgments in utility evaluation of bacteroidales qPCR assays for microbial source tracking in a drinking water source. Environmental Science & Technology. ,vol. 49, pp. 1311- 1318 ,(2015) , 10.1021/ES504579J
Jingrang Lu, Hodon Ryu, Stephen Hill, Mary Schoen, Nicholas Ashbolt, Thomas A. Edge, Jorge Santo Domingo, Distribution and potential significance of a gull fecal marker in urban coastal and riverine areas of southern Ontario, Canada Water Research. ,vol. 45, pp. 3960- 3968 ,(2011) , 10.1016/J.WATRES.2011.05.003