Genetic dissection of vertebrate 53BP1: A major role in non-homologous end joining of DNA double strand breaks
作者： Kyoko Nakamura , Wataru Sakai , Takuo Kawamoto , Ronan T. Bree , Noel F. Lowndes
DOI: 10.1016/J.DNAREP.2006.03.008
关键词:
摘要: Abstract 53BP1 (p53 binding protein) is a BRCT domain-containing protein that rapidly recruited to DNA double strand breaks (DSBs). To investigate the role of in damage response, we generated 53BP1−/− cells from chicken DT40 cell line. As mammalian cells, mutation increased cellular sensitivity ionizing radiation. Although depletion resulted checkpoint defects had normal intra S phase and G2/M checkpoints. G1 specific radiosensitivity higher topoisomerase II suggested defective non-homologous end joining (NHEJ) cells. Genetic analyses confirm this suggestion as have demonstrated an epistatic relationship between NHEJ genes, Ku70 Artemis, but not with Rad54, gene essential for repair DSBs by homologous recombination. We conclude major supporting survival suffered facilitating NHEJ.
elsevier.com
sci-hub.se 参考文章(30)
Enriqueta Riballo, Martin Kühne, Nicole Rief, Aidan Doherty, Graeme C.M. Smith, Marı́a-José Recio, Caroline Reis, Kirsten Dahm, Andreas Fricke, Andrea Krempler, Antony R. Parker, Stephen P. Jackson, Andrew Gennery, Penny A. Jeggo, Markus Löbrich, A Pathway of Double-Strand Break Rejoining Dependent upon ATM, Artemis, and Proteins Locating to γ-H2AX Foci Molecular Cell. ,vol. 16, pp. 715- 724 ,(2004) , 10.1016/J.MOLCEL.2004.10.029
Noritaka Adachi, Sairei So, Hideki Koyama, Loss of Nonhomologous End Joining Confers Camptothecin Resistance in DT40 Cells IMPLICATIONS FOR THE REPAIR OF TOPOISOMERASE I-MEDIATED DNA DAMAGE Journal of Biological Chemistry. ,vol. 279, pp. 37343- 37348 ,(2004) , 10.1074/JBC.M313910200
T. Weinert, L. Hartwell, The RAD9 Gene Controls the Cell Cycle Response to DNA Damage in Saccharomyces cerevisiae Science. ,vol. 241, pp. 317- 322 ,(1988) , 10.1126/SCIENCE.3291120
Noritaka Adachi, Hiromi Suzuki, Susumu Iiizumi, Hideki Koyama, Hypersensitivity of Nonhomologous DNA End-joining Mutants to VP-16 and ICRF-193 Journal of Biological Chemistry. ,vol. 278, pp. 35897- 35902 ,(2003) , 10.1074/JBC.M306500200
SP Lees-Miller, K Meek, Repair of DNA double strand breaks by non-homologous end joining. Biochimie. ,vol. 85, pp. 1161- 1173 ,(2003) , 10.1016/J.BIOCHI.2003.10.011
Yunmei Ma, Ulrich Pannicke, Klaus Schwarz, Michael R. Lieber, Hairpin Opening and Overhang Processing by an Artemis/DNA-Dependent Protein Kinase Complex in Nonhomologous End Joining and V(D)J Recombination Cell. ,vol. 108, pp. 781- 794 ,(2002) , 10.1016/S0092-8674(02)00671-2
Ciaran Morrison, Eiichiro Sonoda, Noriaki Takao, Akira Shinohara, Ken‐ichi Yamamoto, Shunichi Takeda, The controlling role of ATM in homologous recombinational repair of DNA damage The EMBO Journal. ,vol. 19, pp. 463- 471 ,(2000) , 10.1093/EMBOJ/19.3.463
Tatsuo Fukagawa, Yoshikazu Mikami, Ai Nishihashi, Vinciane Regnier, Tokuko Haraguchi, Yasushi Hiraoka, Naoko Sugata, Kazuo Todokoro, William Brown, Toshimichi Ikemura, CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells The EMBO Journal. ,vol. 20, pp. 4603- 4617 ,(2001) , 10.1093/EMBOJ/20.16.4603
Yentram Huyen, Omar Zgheib, Richard A. DiTullio Jr, Vassilis G. Gorgoulis, Panayotis Zacharatos, Tom J. Petty, Emily A. Sheston, Hestia S. Mellert, Elena S. Stavridi, Thanos D. Halazonetis, Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks Nature. ,vol. 432, pp. 406- 411 ,(2004) , 10.1038/NATURE03114
Magda Budzowska, Iris Jaspers, Jeroen Essers, Harm de Waard, Ellen van Drunen, Katsuhiro Hanada, Berna Beverloo, Rudolf W Hendriks, Annelies de Klein, Roland Kanaar, Jan H Hoeijmakers, Alex Maas, Mutation of the mouse Rad17 gene leads to embryonic lethality and reveals a role in DNA damage-dependent recombination The EMBO Journal. ,vol. 23, pp. 3548- 3558 ,(2004) , 10.1038/SJ.EMBOJ.7600353