Biotransformation of carbon tetrachloride in cultured neurons and astrocytes.

摘要: Abstract The ability of brain neuronal cells to metabolize carbon tetrachloride (CCl 4 ) has been studied in an attempt explain earlier observed toxic effects CCl on these cells. expression cytochrome P -450, the glutathione (GSH) content and activity glutathione- S -transferase (GST) were measured cultured neurons astrocytes from chick embryo cerebral hemispheres. metabolism neuron astrocyte cultures was also assessed by determining formation of: 2 membrane preparations In fractions astrocytes, no measurable levels -450 observed. Nevertheless, as well had a capacity for . metabolic significantly greater than that astrocytes. higher initial GSH control GST Neither level nor affected after exposure m slightly depleted induced activity. At 3 , 30% more 50% at It can be concluded activation This observation -induced lipid peroxidation neurons. Moreover, not able protect against peroxidative damage. other hand, appeared have role detoxification