Use of caged fluorochromes to track macromolecular movement in living cells

摘要: Abstract One way to visualize and track the movement of macromolecules in living cell is follow their after tagging molecule with a ‘caged' or chemically masked fluorochrome. The fluorochrome does not fluoresce until caging group released by spot photoactivation, bright fluorescent signal can then be tracked as it moves into dark surrounding area cell. When coupled rapid imaging microscopy, possible measure rates fast macromolecular diffusion. This article describes use photoactivatable fluorochromes intracellular both proteins nucleic acids lineages.