Perspectives on Non-Animal Alternatives for Assessing Sensitization Potential in Allergic Contact Dermatitis.

摘要: Skin sensitization remains a major environmental and occupational health hazard. Animal models have been used as the gold standard method of choice for estimating chemical potential. However, growing international drive consensus minimizing animal usage prompted development in vitro methods to assess sensitivity. In this paper, we examine existing approaches including silico models, cell tissue based assays distinguishing between sensitizers irritants. The that discussed include Quantitative Structure Activity Relationships (QSAR) QSAR expert correlate molecular structure with biological activity mechanism read-across incorporate compound electrophilicity. rely on an assortment mono co-culture systems conjunction 3D skin models. Given complexity allergen induced immune responses, limited ability capture entire gamut cellular events associated these also introduce microfabricated platform can all key steps involved allergic contact Finally, describe integrated testing strategy comprised two or three tier evaluating potential chemicals.