Paired carbon and nitrogen metabolism by ammonia‐oxidizing bacteria and archaea in temperate forest soils

摘要: Nitrification is a biologically mediated nutrient transformation, which influences the availability of inorganic nitrogen to other microorganisms and plants mediates mobility in environment. Ammonia oxidation, rate-limiting step nitrification, performed by two groups microbes: ammonia-oxidizing archaea (AOA) bacteria (AOB) that couple this process with chemoautotrophic fixation carbon. Due energetic constraints on these organisms, both AOA AOB likely oxidize large amounts ammonia fix relatively small carbon natural environments. Here we sought investigate paired metabolism forest soils. To accomplish objective, used quantitative polymerase chain reaction (qPCR) quantify changes monooxygenase subunit A (amoA) genes during situ incubations. We then qPCR data alongside community profiles at each site convert amoA gene copy number accumulation group. Finally, regressed group-specific values against observed NO3− establish cross-site relationships between oxidation By procedure estimated soil oxidized 59.8 μg ammonia-N add 1 biomass, while 58.2 biomass. These findings represent first field-based estimates could be inform microbially explicit models nitrification