Biosurfactant Production by Marine-Originated Bacteria Bacillus Subtilis and Its Application for Crude Oil Removal

作者: Zhiwen Zhu , Baiyu Zhang , Bing Chen , Qinghong Cai , Weiyun Lin

DOI: 10.1007/S11270-016-3012-Y

关键词:

摘要: Biosurfactants have been considered as promising candidates for oil spill cleanup they are generally more biodegradable, less toxic, and better in enhancing biodegradation than chemical surfactants. This study targeted the marine microbial biosurfactants to examine their enhanced production methods application removal of crude from soil. The generated by Bacillus subtilis, which was isolated Atlantic Ocean, were investigated this study. economic medium using different carbon (n-hexadecane, diesel oil, glycerol, glucose, starch, sucrose) nitrogen sources (NaNO3, (NH4)2SO4, yeast extract) studied. best performance biosurfactant achieved when glycerol source sodium nitrate extract substrate. rate five times compared with that original screening recipe. fermentative could reduce surface tension water 27 mN/m strong activity (∼36.4 mN/m) even after dilution 10 times. critical micellar concentration (CMC) product 507 mg/L. A thin layer chromatography (TLC) analysis indicated purified a mixture lipopeptide glycolipid. microbially produced further examined soil-washing agent enhance soil column system. rates 58 65 % solution concentrations 4 8 g/L, respectively. results demonstrated potential cleaning oil-contaminated

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