Rapid and simple protein-stability screens : application to membrane proteins

摘要: Approximately 30% of the human genome, and likewise for other genomes, encodes membrane proteins. Also, majority known pharmaceutical targets are As a consequence, future success structure-based drug-design efforts will rely heavily on membrane-protein structural information. While number techniques available to determine structure proteins, crystallographic methods (either using two-dimensional or three-dimensional crystals) have been most productive. Nonetheless, determination has encountered at least three serious bottlenecks: protein production, purification crystallization. crystallization strategies proteins today, they all must ensure that interest is thermodynamically stable be feasible. Thermodynamic stability so fundamental it often overlooked experimentally. Here, simple effective protocols determining relative stabilities commercially instruments reagents demonstrated. The results demonstrate suitability rapid screening conditions maximize minimal amounts protein.