An injectable extracellular matrix for the reconstruction of epidural fat and the prevention of epidural fibrosis

摘要: Extensive epidural fibrosis is a common complication following spinal surgery and can cause pain limited mobility. In the present study, novel biomimetic approach was developed to prevent postsurgical adhesion of dura. We aimed reconstruct fat, which prevents scar-tissue adhesion, through development an injectable decellularized adipose matrix (DAM)-containing hyaluronic acid (HA) hydrogel loaded with stromal cells (ASCs). Injectable DAM prepared from porcine tissue by four freeze-thaw cycles subsequent pepsin digestion. Residual analyses confirmed efficacy detergent-free decellularization, while most sulfated glycosaminoglycans collagen were preserved. The Transwell migration assay demonstrated anti-infiltrative property DAM-containing HA hydrogel. After 14 d 3D culture, showed inductive potential in adipogenic differentiation ASCs. For vivo ASC-loaded (DAM/ASC-incorporated hydrogel) injected into adult laminectomized male rats, results assessed microscopic histological examination. data indicated that hydrogel, DAM, ASCs all required for ability engineered fat block invasion fibrous tissue. Our suggested this DAM/ASC-incorporated has applications minimally invasive soft-tissue reconstruction prevention.