Construction and symbiotic competence of a luxA-deletion mutant of Vibrio fischeri

摘要: Bioluminescence by the squid Euprymna scolopes requires colonization of its light organ symbiotic luminous bacterium Vibrio fischeri. Investigation genetic determinants underlying bacterial competence in this system has necessitated continuing establishment and application molecular techniques V. We developed a procedure for introduction plasmid DNA into fischeri electroporation, isolated mutant strain that overcame apparent restriction barrier between Escherichia coli. Using technique electroporation combination with gene replacement, we constructed non-luminous (delta luxA::erm). In addition, used transducing phage rp-1 first time to transfer chromosomal antibiotic resistance marker another The luxA was able colonize E. as quickly same extent wild type. This result suggested that, at least during initial stages colonization, luminescence per se is not an essential factor infection.