Opposite Function of ERα and ERβ in Controlling 17β-Estradiol-mediated Osteogenesis in Osteoblasts.

摘要: Estrogen receptor plays critical roles in osteogenesis but the underlying mechanism remains unclear. In order to determine effect of ERα and ERβ on several factors regulating human osteoblasts. Cell based assy, RT-PCR immunoblot analyses were used research. Both showed that gene expression OPG, MBP2, TGF-β, RUNX2, IGF-1 was significantly reduced while RANKL drastically increased after shRNA-based depletion MG-63 Surprisingly, 17β-estradiol (E2) treatment led remarkably compared with E2 untreated cells. contrast, an opposite role RANKL. However, double could not rescue these vitro. Our results provide a novel estrogen controlling cells as well potential clinic therapeutic target osteoporosis.