Distinct epitopes on Ik gene products identified by monoclonal antibodies

摘要: Analysis of reactivity monoclonal anti-Iak alloantibodies, obtained by fusion between NS 1 myeloma and spleen cells from alloimmune A.TH mice, permitted the identification 9 distinct determinants Ik gene products. Competitive binding experiments indicated that 2 private epitopes (defined H 8–109.13 8–138.4 antibodies) I-Ak product could be separated, thereby apparently splitting Ia.2 specificity. A public determinant molecule (identified 8–15.9 antibody) was found expressed not only on 1-A products H-2b, H-2d, H-2ja, H-2P H-2q murine haplotypes, but also human HLA-DR antigens. Four I-E/Ck antigen 7–8.26, 10–81.10, 10–93.2 8–86.2 had a strain distribution analogous to Ia.7 However, competitive experiments, cross-reactivity pattern with la-like antigens other species (e.g. antigens) these antibodies detected molecule, subdividing broad Two H9–14.8 H9–15.4 did permit precise assignment given Ia antigen, even though preliminary data suggested they detect separate product. All identified membrane expected tissue pattern, most them precipitate containing two chains 28 kD 35 kD, mouse cell lysates.