Intracellular Transport of a Karyophilic Protein

摘要: The term ‘karyophilic’ was suggested (Dabauvalle and Franke, 1982) for cellular proteins which accumulate in the nucleus without binding to chromatin or other nuclear structures. Absence of means that these are recovered from supernatant centrifuged cell homogenates. classification originated studies Xenopus oocytes (Bonner, 1975;; Merriam Hill, 1976; De Robertis et al., 1978). exceptionally large size cells permits manipulations not easily applied average-sized somatic cells. For instance, can be loaded with macromolecules by microinjection, they fractioned particularly fast mild manual procedures, frequently fractions even single enough analyzed centrifugation gel electrophoresis. With methods several karyophilic oocyte were characterized designated as N1-N4 nucleoplasmin. Karyophilic also present amphibians than 1982). However, their occurance is largely unresolved.