Molecular characteristics, antibiogram and prevalence of multi-drug resistant Staphylococcus aureus (MDRSA) isolated from milk obtained from culled dairy cows and from cows with acute clinical mastitis

摘要: Abstract Objectives To study the molecular characteristics, antibiogram and prevalence of multi-drug resistant Staphylococcus aureus ( S. aureus ) (MDRSA) isolated from milk obtained culled dairy cows with acute clinical mastitis. Methods Bacteria were cultured 188 quarter samples before culling n  = 139) affected mastitis  = 49) belonging to 10 farms. The bacteria identified using colony morphology, Gram staining biochemical characteristics. isolates then subjected characterization PCR targeting 16S rRNA mecA gene identify Methicillin (MRSA). all was performed Kirby–Bauer disk diffusion method against commonly used antibiotics in Results 19 (13.7%) 11 (22.4%) In both mastitis, in vitro revealed that 100% erythromycin, penicillin G, streptomycin, doxycyclin, trimethoprim/sulpha. MRSA 26.3% 18.2%, respectively, an overall 3.7% among samples. All completely tested antibiotics. positive for presence gene. Conclusions carrying mastitic Jordan first time. may pose a potential health risk public, farm workers veterinarians.