[Deletion mutants of human granulocyte-macrophage colony-stimulating factor].

摘要: To study the structure-function relationship of human granulocyte-macrophage colony-stimulating factor (GM-CSF), genes were constructed that encode its three deletion mutants: D1, a mutant with six amino acid residues (37-42) some which are part beta-structural region; D2, unstructured six-aa sequence loop (45-50); and D3, 14 aa (37-50) corresponding to A-B encoded by second exon gmcsf gene. The expression products these in E. coli accumulated fraction insoluble proteins. secondary structures proteins similar full-size GM-CSF, but biological activity mutants was 130 times lower than GM-CSF: they stimulated proliferation TF-1 cell line at 3 ng/ml concentration. resulting displayed antagonistic properties toward inhibition degree being 27%. A decrease row D2 > D1 D3 implies importance conserved hydrophobic involved formation beta-structure for GM-CSF functional conformation.