Rainbow trout and human cells in culture for the evaluation of the toxicity of aquatic pollutants: A study with cadmium☆

摘要: Abstract Rainbow trout (RTG-2) and human skin epithelial cells (NCTC 2544) were tested as bioassay organisms for the evaluation of toxicity aquatic pollutants. Cadmium (Cd) was used a representative model compound. The cell lines grown monolayers at 15 37°C in minimal essential medium supplemented with 10% fetal bovine serum (FBS). RTG-2 NCTC 2544 exposed to 100 ppb 2.4 ppm Cd. Exposure Cd maintained period time sufficient an inoculum 105 or 5 × 104 reach confluence controls 60-mm Petri dish: 20 days 9 2544. Every 2 every day renewed 3 dishes each group determination following parameters: total protein, DNA, RNA content incorporation [3H]thymidine DNA [14C]uridine RNA. cytotoxicity apparent only when concentration reduced. With 1% FBS induced dramatic inhibition increase protein cells. At ppm, these effects seen early (protein RNA) after added cultures. labelled precursors equally much lower treated samples (2.4 ppm) compared controls. ppb, affected; effect 10 exposure persisted until end experiment. appeared be more sensitive cadmium than cells, severe depression all parameters observed. However, contrasting significant differences measured least on 4 (protein, [14C] incorporation). data are those previously obtained lead using similar system. results also analysed light available vivo attempt establish correlation between vitro responses. This is considered important step process validation present