Use of nitrate non-utilising (Nit) mutants to determine vegetative compatibility in Botryotinia fuckeliana (Botrytis cinerea)
作者： Ross E. Beever , Stephanie L. Parkes
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摘要: Nitrate non-utilising (Nit) mutants of six field strains and three single-ascospore Botryotinia fuckeliana were selected by plating mycelial plugs onto a nitrate-containing minimal medium amended with chlorate. Mutants characterised growth responses on various sole nitrogen sources. For each parental strain two produced: nit1, defective in nitrate reductase apoenzyme; NitM, the molybdenum cofactor pathway. Complementation was observed between nit1 NitM from same parent all instances. However, complementation not any such combinations when derived different parents. It is concluded that vegetative incompatibility system operates B. resulting multiple compatibility groups. One cross strains, indicating new groups can be generated during sexual reproduction. Mycelial interaction zones formed they paired NaCl-amended medium, congruence incompatibility.
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