SV40‐induced transformation of human diploid cells: Crisis and recovery

摘要: Nineteen cultures of human embryonic or adult-tissue cells were exposed to SV40 as primary developed cell strains in Phase II cultural life; 12 virus III, at the end vitro life. After expected proliferative response, infected exhibited morphological change, accelerated growth, and prolongation propagability. an average 22.6 weeks for explants II, stage transformation ended a “crisis.” Crisis was characterized by progressive decline vigor culture proliferation, increasingly abnormal division, limitation propagability eventually resulting diminution number. Cytological features crisis included detachment appearance multinucleated giant cells. Temporally cytologically, similar transformed whether adult origin, derived from skin, buccal mucosa lung, III life time infection (the “older” entering sooner than “younger”), propagated continuously with intervening periods growth arrest proliferation vivo. variable “recovery” occurred repopulation small groups surviving but seemingly dormant The new populations, always obtained care crisis, formed propagable lines. Control uninfected have finite terminating which is characteristic diploid strains. Passage through recovery accompanied loss capacity release infectious enhancement production SV40-induced complement-fixing antigen.