Keynote Lecture

摘要: Resveratrol and its methylated, glucosylated and oligomerized derivatives act in plants both as allelochemicals and as phytoalexins taking part in their defense mechanisms [1]. These compounds are especially produced by grape tissues upon fungal infection or stress [2]. For the study of the role of hydroxystilbenes in plant defense mechanisms, analyzing these compounds in plant tissues is needed. Resveratrol and most of its derivatives with the exception of some trimeric compounds are conjugated systems easily detected in UV and by fluorimetry. Resveratrol and derivatives have thus been analysed following extraction from plant tissues using various HPLC methods coupling diode array detection and fluorimetry [3]. HPLC has also been associated with tandem mass spectrometry for the analysis of hydroxystilbenes [4]. For the elucidation of resveratrol oligomers, relatively complex analytical systems are necessary: advanced mass spectrometry techniques and extensive one-and two-dimensional nuclear magnetic resonance measurements, UV, CD, optical properties, and molecular mechanic calculations were used to the identification of stilbenes extracted following a multi-step purification process from various Vitis vinifera genotype leaves infected with Plasmopara viticola [5]. Hydroxystilbenes have also been detected and quantified by direct analysis in both Botrytis cinerea infected and non-infected tissues by using analytical techniques incorporating laser desorption coupled with laser resonant ionization and time-offlight mass spectrometry [6]. Very recently, suspect screening analysis, which is a targeted metabolomics approach, has …