IDENTIFICATION OF A LIPASE INVOLVED IN JASMONIC ACID BIOSYNTHESIS IN NICOTIANA ATTENUATA

摘要: Jasmonic acid (JA) is a multifunctional growth regulator widely distributed in the plant kingdom that modulates anther dehiscence, fruit ripening, root growth, tendril coiling, and plant resistance to insects and pathogens. The first step of JA biosynthesis is catalyzed by a lipase that hydrolyzes plastidial membrane lipids releasing free fatty acids. The identification of two Arabidopsis plastidial phospholipases, DAD1 and DGL provided genetic evidence that this is a critical step in the activation of JA biosynthesis. We identified three putative functional homologues of DAD1 and DGL in N. attenuata: PLA1, PLA2 and PLA3 that were functionally characterized by Virus Induced Gene Silencing (VIGS) to investigate their involvement in JA biosynthesis. After elicitation by wound and fatty acids coniugates (FACs), leaves of PLA2 and PLA3 VIGS-silenced plants accumulated similar amounts of JA and JA-Isoleucine (JA-Ile …