Tracking of single nanoparticles in living cells

摘要: High molecular proteins, such as membrane proteins, play fundamental roles in many physiological processes and are target proteins for drug development. For better understanding of the functions of the proteins, not only precise static three-dimensional structures determined by X-ray crystallography and cryo-electron microscopy methodologies, but also dynamical nature are required. NMR (nuclear magnetic resonance spectroscopy) provides us information about proteins dynamics, including conformation equilibrium related to functions. However, it is frequently difficult to obtain information about the protein dynamics related to the functions, due to the molecular weight limitation in NMR. We have recently developed novel NMR methods for characterizing protein dynamics utilizing multiple quantum relaxation rates of side-chain methyl groups, which can be sensitively observed in high molecular weight proteins. In this paper, we will show our recent results of function-related dynamics of high molecular proteins.