MEMBRANE TRANSPORT, STRUCTURE, FUNCTION, AND BIOGENESIS-Direct Evidence for Substrate-induced Proton Release in Detergent-solubilized EmrE, a Multidrag Transporter.

摘要: A novel approach to study coupling of substrate and ion fluxes is presented. EmrE is an H+-coupled multidrug transporter from Escherichia coli. Detergent-solubilized EmrE binds substrate with high affinity in a pH-dependent mode. Here we show, for the first time in an ion-coupled transporter, substrate-induced release of protons in a detergent-solubilized preparation. The direct measurements allow for an important quantitation of the phenomenon. Thus, stoichiometry of the release in the wild type and a mutant with a single carboxyl at position 14 is very similar and about 0.8 protons/monomer. The findings demonstrate that the only residue involved in proton release is a highly conserved membrane-embedded glutamate (Glu-14) and that all the Glu-14 residues in the EmrE functional oligomer participate in proton release. Furthermore, from the pH dependence of the release we determined the pK of Glu-14 as 8.5 …