Sputtered Fiducial Markers for Combined SIMS and MALDI Imaging

摘要: Mass spectrometry imaging (MSI) is capable of providing simultaneous identification and localization of biomolecules without the need for any labels. To overcome the limitations of a single MSI technique a multimodal approach is needed to study the complexity of biological tissue samples [1]. Secondary Ion Mass Spectrometry (SIMS) allows for high spatial resolution imaging while Matrix-Assisted Laser Desorption Ionization (MALDI) offers a significantly wider mass range. The combination of overlaid SIMS and MALDI images results in detailed and unique biomolecular information [1-3]. A proper co-registration of the two imaging modalities requires accurate fiducial markers (FM)[4]. We developed a new FM strategy for MALDI and SIMS co-registration.