HISTONE NEIGHBORS AND THE STRUCTURE OF CHROMATIN.

摘要: Materials and Methods.................................................. 24Materials........................................................... 24Preparation of Nuclei and Chromatin................................. 24Preparation of Chromatin Depleted of HI, H2a, and H2b.... 25vPageConcentration and Mass of the Chromatin............................ 27ReactionwithDimethylsuberimidateandDimethyladipimidate. 27ReactionwithMethyl-4-Mercaptobutyrimidate...................... 30Reactionwitha Carbodiimide..................................... 31ReactionwithGlutaraldehyde................................... 31Polyacrylamide Gel Electrophoresis................................. 31Results.............................................................. 32Diversity of Reagents Producing Cross-Links........................ 32Diimidoester Cross-Linking as a Function of IonicStrength and pH.................................................... 37Diimidoester Reaction in Chromatin Depleted ofSelected Histones.................................................. 51Carbodiimide Reaction …