Transient Receptor Potential Ankyrin-1 Deficiency in Mice Protected Against Myocardial Ischemia-Reperfusion Injury

摘要: Introduction: Myocardial ischemia-reperfusion (I/R) results in the generation of oxygen-derived free radicals, the accumulation of lipid peroxidation-derived unsaturated aldehydes, variable angina (ischemic pain) and infarction. Pain receptors known as transient receptor potential ankyrin-1 (TRPA1) are activated by endogenous aldehydes, including acrolein and 4-hydroxynonenal, yet the contribution of TRPA1 (a calcium permeable channel) to I/R-induced myocardial injury has not been assessed. Hypothesis: We tested the hypothesis that the absence of TRPA1 would affect I/R injury. Methods and Results: Adult male C57BL/6 mice hearts and isolated cardiomyocytes were positively stained with an immunofluorescent antibody to TRPA1 with intense localization at intercalated disks. Presence of mRNA for human TRPA1 and mTrpa1 was confirmed by qRT-PCR in cardiomyocytes from human induced pluripotent …