Yeast two-hybrid analysis of skeletal muscle triad junction proteins

摘要: Excitation-contraction (EC) coupling in muscle is the process by which electrical stimulation releases calcium from the sarcoplasmic reticulum (SR), thereby allowing cross-bridge cycling between actin and myosin to occur. EC coupling occurs at specialized membrane regions called triad junctions. In this dissertation, the yeast two-hybrid system was used to search for interactions among known and novel proteins of triad junctions. In direct interaction tests, no strong interaction was detected between the dihydropyridine receptor (DHPR) II–III loop and the skeletal muscle ryanodine receptor (RyR); however, a possible weak interaction between physiologically important parts of the two receptors was investigated. In screens of a skeletal muscle cDNA library, no interactors were detected for the N-terminus, the I–II, II–III, or III–IV loops of the skeletal muscle DHPR. Some potential interactors of s53, of RyR1 and of junctin …