Normalization and systematic measurement error in cDNA microarray data

摘要: Data collected from cDNA Microarrays, like all data from techniques have some measurement error. However cDNA much of the measurement in cDNAs is not random or normally distributed but systematic. Physical parameters inherent in the production of microarrays can be used to approximately measure these systematic effects. Although the causes of these effects are not fully understood their influence can be corrected mathematically. By building a model of gene expression, which is based on a few fundamental assumptions, a method for correction was developed and is presented here. As long as the assumptions are valid, the methods for correcting data should substantially reduce measurement error