Brief history of yeast genomics

作者: Stacia R Engel , J Michael Cherry

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摘要: A diverse set of Saccharomyces cerevisiae genomes have been sequenced, encompassing a variety of commercial and laboratory strains, as well as wild isolates, many of which have been made available from the Saccharomyces Genome Database (SGD). Here we present a description of the isolation and uses of these budding yeast strains, their current incorporation into SGD and our plans for future developments in their annotation and analysis. The first completed eukaryotic genome sequence was that of the yeast S. cerevisiae strain S288C, completed through the effort of a worldwide sequencing consortium (1). S288C has a complex genealogy, but is derived primarily ($88% of its genome) from strain EM93, which was isolated from a rotting fig in Central California in 1938 (2). The remaining 12% of the S288C genome comes from five different progenitors: two natural isolates (EM126 isolated in 1939 also from a rotting fig in Central California, and NRRL YB-210 isolated from rotting bananas from Costa Rica in 1942) and three commercial baking strains (Yeast Foam, FLD and LK). S288C is a widely used laboratory strain, designed by Mortimer for biochemical studies, and specifically selected to be non-flocculent with a minimal set

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