In-vitro Contractility of Normal Human Vermiform Appendix involves 5-Hydroxytryptamine (5-HT3) Pathways in addition to Muscarinic Transmission.

摘要: Appendicitis is a cause of health concern in all age and sex worldwide. Alteration in appendicular peristalsis/contractility has been implicated as a precipitating factor in appendicitis. But, the neurotransmitters involved in the contractility of normal human vermiform appendix are not well understood. The present study was undertaken to investigate the neurotransmitter mediated pathways involved in mediating the contractility of longitudinal muscles of normal human vermiform appendix. Longitudinal muscle strips of human vermiform appendix were mounted in Dale's organ bath. In vitro contractility to agonist was recorded using Statham's isometric force displacement transducer. Experiments were performed to determine the dose-response of agonists (acetylcholine/5-HT/histamine) and the dose that produced maximum contractions. In a separate set of experiments, the effect of antagonists (like atropine …