Proton NMR studies of the glucocorticoid receptor DNA-binding domain: sequential assignments and identification of secondary structure elements

摘要: Materials and MethodsThe two protein fragments containing the GRDBD (Figure 1) were KPARP (C440-G525) RL (DBD-93, numbering re-ferring to the rat GR), which was purified and checked for sequence-specific DNA binding activity as described by