Cyclooxygenase-two regulates proliferation in aggressive fibromatosis (desmoid tumor)

摘要: Methods: Eighteen cases of sporadic aggressive fibromatoses were studied. Cryopreserved tumor tissue for RNA analysis was available from all 18, and primary explant cell cultures were available from four. For eight of the cases, cryopreserved marginal" normal" tissues also were available, and in six of the cases, large enough quantities of cryopreserved tissues were available for Northern and Western blot. In four cases primary cell cultures were established.RT-PCR using specific oligonucleotide primers for COX-2 and GAPDH (housekeeping control) were used to test for COX-2 expression. Northern blot, using a PCR generated digoxigenin labeled probe was performed in six cases of tumor tissue, and corresponding" normal" marginal tissues. Western blot was also performed, using a commercially available polyclonal antibody (Oxford Biomedical, PG 27) on six of the tumor tissues. Primary cell cultures were …