Gas-phase purification enables accurate, large-scale, multiplexed proteome quantification

摘要: We describe a mass spectrometry method, QuantMode, which improves the accuracy of isobaric tag–based quantification by alleviating the pervasive problem of precursor interference—co-isolation of impurities—through gas-phase purification. Analysis of a model interference sample with a 10: 1 ratio improved from 4.4: 1 to 8.5: 1, with a minimal loss of spectral identifications (21%). This technique substantially improves the accuracy of multiplexed quantitative proteomics with isobaric tagging.