Hybridization to high-density filter arrays of a Brugia malayi BAC library with biotinylated oligonucleotides and PCR products

摘要: The development of chemiluminescent detection has provided a rapid, nonradioactive method for positional mapping to cDNA or genomic libraries gridded onto high-density filter arrays (6, 14), Southern and northern blotting, plaque lifts, colony hybridizations, and DNA sequencing (4, 5, 7, 11, 12). These nonradioactive methods are being used for mapping the genome of Brugia malayi, a parasitic nematode and causative agent of lymphatic filariasis. This disease affects over 120 million people worldwide, with over a billion people at risk from infection from three main species of filarial nematodes (3, 9, 15). A full description of the project is provided at http://neb. com/fgn/filgen1. html. More than 22000 expressed sequence tags (ESTs) representing over 8000 different genes have been sequenced from cDNA libraries constructed from various life-cycle stages. For mapping these genes to the B. malayi genome …