Mitophagy suppression by miquelianin‐rich lotus leaf extract induces ‘beiging’of white fat via AMPK/DRP1‐PINK1/PARKIN signaling axis

摘要: Background Lotus (Nelumbo nucifera) leaf has been described to have anti‐obesity activity, but the role of white fat ‘browning’ or ‘beiging’ in its beneficial metabolic actions remains unclear. Here, 3T3‐L1 cells and high‐fat‐diet (HFD)‐fed mice were used to evaluate the effects of miquelianin‐rich lotus leaf extract (LLE) on white‐to‐beige fat conversion and its regulatory mechanisms. Results Treatment with LLE increased mitochondrial abundance, mitochondrial membrane potential and NAD+/NADH ratio in 3T3‐L1 cells, suggesting its potential in promoting mitochondrial activity. qPCR and/or western blotting analysis confirmed that LLE induced the expression of beige fat‐enriched gene signatures (e.g. Sirt1, Cidea, Dio2, Prdm16, Ucp1, Cd40, Cd137, Cited1) and mitochondrial biogenesis‐related markers (e.g. Nrf1, Cox2, Cox7a, Tfam) in 3T3‐L1 cells and inguinal white adipose tissue of HFD‐fed mice …