Single nucleotide polymorphism in beta2-adrenoceptor gene and the distribution in Chinese Han ethnic group

摘要: ObjectiveThe study was conducted to investigate single nucleotide polymorphism (SNP) in beta2-adrenoceptor (beta2-AR) gene and the distribution of these identified SNPs in Chinese Han ethnic group.Methodsbeta2-AR gene was sequenced to detect SNPs by fluorescent labeling automatic sequencing method in 80 unrelated samples from territory of Dabie Mountain in Anhui province.ResultsA total of 8 SNPs were identified in length of 3.8 kb, including 5 SNPs in code region, 3 SNPs in regulatory region. Although the variations,-468C to G,-367T to C,-47C to T,-20T to C,+ 79C to G,+ 100G to A,+ 491C to T,+ 1098T to C have been identified in other ethnic groups, they have not been found in our study. The allele distribution of SNPs is in good unity with the Hardy-Weinberg equilibrium.ConclusionThe distribution of SNPs in beta2-AR gene is not equable and the SNPs in different ethnic groups differ greatly. The allele distribution of SNPs conforms well to the Hardy-Weinberg equilibrium.