Genotoxic effects and oxidative stress responses induced by retene in marine mussels(Mytilus galloprovincialis)

摘要: Mediterranean mussels (Mytilus galloprovincialis Lmk.) were exposed to 0 (control) and 2.7 mu M 7-isopropyl-1-methylphenanthrene (Retene) for 6, 12, 18 and 24 hours. Reduced glutathione (GSH), glutathione S-transferase (GST) activity, glutathione peroxidase (GPx) activity, catalase (CAT) activity, lipid peroxidation (LPO) and DNA strand-breaks were measured in mussel gills and hepatopancreas. Retene induced a significant inhibition of gill GST and GPx activities at 18 hours, whereas GSH was significantly increased at 18 hours. Gill CAT activity was also inhibited at 24 hours exposure. Gill LPO was slightly increased at 24 hours, whereas DNA integrity was not altered during retene exposure. Mussel hepatopancreas GPx activity was significantly inhibited at 6 hours exposure to retene, whereas GSH significantly increased at 12 hours and DNA integrity decreased at 12 and 24 hours. The hepatopancreas DNA …