Genetic Background of Dalmatian Pyrethrum (Tanacetum cinerariifolium/Trevir./Sch. Bip.) Insecticidal Potential

摘要: Pyrethrin is the secondary metabolite synthesized in Dalmatian pyrethrum (Tanacetum cinerariifolium/Trevir./Sch. Bip.) and recognized for its potent insecticidal activity with no adverse effects on humans and environment. The natural distribution area of Dalmatian pyrethrum expands throughout the Eastern Adriatic coast and the islands. Today, as an endemic species, Dalmatian pyrethrum is threatened by anthropogenic habitat loss and degradation related to factors such as urbanization and habitat conversion and therefore protected by the Ordinance on Strictly Protected Species (Official Gazette 144/13, 73/2016). Despite its historical significance and economic value limited genetic and biochemical data on Dalmatian pyrethrum is available. Therefore, the importance of this project proposal lies in the integration of various scientific fields in resolving important issues concerning genetic and biochemical diversity of Dalmatian pyrethrum. The scientific objectives of the project are:(i) development of microsatellite primers (SSR; Simple Sequence Repeats) for Dalmatian pyrethrum;(ii) utilization of newly developed SSR and Amplified Fragment Length Polymorphism (AFLP) markers in assessing genetic diversity of D. pyrethrum natural populations (iii) optimization of Matric Solid Phase Dispersion (MSPD) technique and its utilization in the extraction of pyrethrins, and identification and determination of six extracted pyrethrin components in sampled populations with High-Performance Liquid Chromatography-DAD (HPLC-DAD),(iv) identification of AFLP markers linked to the trait loci related to the content of each pyrethrin component through …