Dose-and Time-dependent Effects of Sub-chronic Functionalized MWCNT Exposure on Human Mesothelial Cell Neoplastic Transformation Potential

摘要: Comparative studies evaluating how MWCNT physicochemical properties influence pleural mesothelioma potential are critically needed. We hypothesized that MWCNT surface functionalization would affect human mesothelial cell neoplastic transformation potential in a dose/time dependent manner. Cells were continuously exposed to fully characterized parent (p), two different functionalized (f) MWCNTs, or asbestos (ASB) for 6 months at in vivo-relevant doses. Low dose fMWCNT caused significant cell proliferation increase compared to controls throughout the exposure. pMWCNT, one fMWCNT and ASB cells exhibited significantly greater numbers of soft agar colonies compared to controls, with low dose eliciting a more potent effect. Cells exposed to only one fMWCNT exhibited a significant increase in cell invasion ability. Subchronic in vitro exposure models can assist in screening and prioritizing engineered …