Multiple-Diguanylate Cyclase-Coordinated regulation of pyoverdine synthesis in Pseudomonas aeruginosa

摘要: The nucleotide signalling molecule bis‐(3'‐5’)‐cyclic dimeric guanosine monophosphate (c‐di‐GMP) plays an essential role in regulating microbial virulence and biofilm formation. C‐di‐GMP is synthesized by diguanylate cyclase (DGC) enzymes and degraded by phosphodiesterase (PDE) enzymes. One intrinsic feature of c‐di‐GMP signalling is the abundance of DGCs and PDEs encoded by many bacterial species. It is unclear whether the different DGCs or PDEs coordinately establish the c‐di‐GMP regulation or function independently of each other. Here, we provide evidence that multiple DGCs are involved in regulation of c‐di‐GMP on synthesis of the major iron siderophore pyoverdine in Pseudomonas aeruginosa. Constitutive expression of the WspG or YedQ DGC in P. aeruginosa is able to induce its pyoverdine synthesis. Induction of pyoverdine synthesis by high intracellular c‐di‐GMP depends on the …