Abstracts of the Annual Meeting of the Iuliu Haţieganu, University of Medicine and Pharmacy: 3rd–7th December 2018, Cluj-Napoca

摘要: Materials and methodsIn vitro studies were performed on HUVEC cell line, using TMW and TMC in two different dilutions (1/10000, 1/100000), under normoglycemic and hyperglycemic conditions. NF-kB, activated pNF-kB, HIF 1α and γH2AX were assessed by western blot and MDA levels by spectrofluorimetry. 36 Wistar rats were used for the in vivo study. The animals were divided in 4 groups (n= 9/group): Control (Carboxymethylcellulose, CMC), DM, DM+ TMW, DM+ TMC. TMW and TMC (200 mg/kg bw) were orally administered for 14 days. On the 15th day, one dose of STZ (30 mg/kg bw) was intraperitoneally administered. Subsequently, natural compounds were administrated for the next 14 days. On the 33rd day, Open Field Test and Elevated Plus Maze were performed. Oxidative stress biomarkers in serum, hippocampus and frontal lobe homogenates (MDA, GSH/GSSG) and NF-kB levels in hippocampus and frontal lobe samples were also assessed. Methyl CpG binding protein (MECP) 2 and histone deacetylase 1 (HDAC1) expressions in rats’ brain were also analyzed by western blot.ResultsIn vitro, TMW and TMC diminished MDA, NF-kB and γH2AX levels and increased pNF-kB and HIF 1α expressions. In vivo, TMW and TMC administration reduced blood glucose levels, improved the overall mobility and increased 5 times the entrances and time spent in the open arms in EPM. In frontal lobe, both extract diminished lipid peroxidation and enhanced the antioxidant capacity and HDAC1 expression. TMW administration increased NF-kB level and diminished MECP2 expression in hippocampus.ConclusionsBoth compounds exerted …